
Antibodies | Assay Kits | Biology Cells | cDNA | Clia Kits | Culture Cells | Devices | DNA Templates | DNA Testing | Elisa Kits | Enzymes | Exosomes | Gels | Isotypes | Medium & Serums | NATtrol | Panel | Particles | PCR | Pcr Kits | Peptides | Reagents | Recombinant Proteins | Ria Kits | RNA | Test Kits | Vector & Virus | Western Blot
Best Recombinant Human Isocitrate dehydrogenase
Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase.
- Mycobacterium tuberculosis (Mtb) has tailored its metabolism for persistence within the human macrophage. The diversifications are more likely to contain Mtb’s core middleman metabolism, whose enzymes have been little studied. The tricarboxylic acid cycle is predicted to yield precursors for vitality, lipids, amino acids, and heme.
- The genome sequence of Mtb H37Rv predicts the presence of a whole tricarboxylic acid cycle, however we not too long ago discovered that alpha-ketoglutarate dehydrogenase (KDH) exercise is missing in Mtb lysates.
Clostridium difficile Toxin A Antibody
- Right here we confirmed that citrate synthase, aconitase, isocitrate dehydrogenase, fumarase, malate dehydrogenase, and succinate dehydrogenase, however not KDH, are current, elevating the potential for separate oxidative and reductive half-cycles.
- As a possible hyperlink between the half-cycles, we discovered that Rv1248c, annotated as encoding SucA, the putative E1 part of KDH, as an alternative encodes alpha-ketoglutarate decarboxylase (Kgd) and produces succinic semialdehyde. Succinic semialdehyde dehydrogenase exercise was detected in Mtb lysates and recapitulated with recombinant proteins GabD1 (encoded by Rv0234c) and GabD2 (encoded by Rv1731).
DNA Polymerase for PAGE (with 2.5 mM dNTPs) Enzyme
- Kgd and GabD1 or GabD2 type an alternate pathway from alpha-ketoglutarate to succinate. Rv1248c, which is crucial or required for regular development of Mtb [Sassetti, C., Boyd, D. H. & Rubin, E. J. (2003) Mol. Microbiol 48, 77-84] is the primary gene proven to encode a Kgd. Kgd is missing in people and will signify a possible goal for chemotherapy of tuberculosis.
![]() NATtrol Clostridium difficile NAP1, External Run Control, Medium (6 X 1 mL) |
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NATCDI(NAP1)-ERCM | Zeptometrix | 6 X 1 mL | EUR 499.28 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
![]() Nap1/ Rat Nap1 ELISA Kit |
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ELI-22779r | Lifescience Market | 96 Tests | EUR 886 |
![]() CLOSTRIDIUM DIFFICILE AGAR |
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C03-109-10kg | Alphabiosciences | 10 kg | EUR 1745 |
![]() CLOSTRIDIUM DIFFICILE AGAR |
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C03-109-2kg | Alphabiosciences | 2kg | EUR 418 |
![]() CLOSTRIDIUM DIFFICILE AGAR |
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C03-109-500g | Alphabiosciences | 500 g | EUR 150 |
![]() Clostridium difficile antibody |
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10-2724 | Fitzgerald | 1 mg | EUR 424 |
Description: Mouse monoclonal Clostridium difficile antibody (GDH specific) |
![]() NAP1 Antibody |
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20-abx008064 | Abbexa |
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![]() NAP1 siRNA |
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20-abx925398 | Abbexa |
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![]() NAP1 siRNA |
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20-abx925399 | Abbexa |
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![]() NAP1 antibody |
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70R-51074 | Fitzgerald | 100 ul | EUR 244 |
Description: Purified Polyclonal NAP1 antibody |
![]() Clostridium difficile Toxoid A |
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abx670094-50ug | Abbexa | 50 ug | EUR 1052 |
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![]() Clostridium difficile Toxoid B |
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abx670095-50ug | Abbexa | 50 ug | EUR 1052 |
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![]() Clostridium difficile PCR kit |
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PCR-VH005-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Clostridium difficile |
![]() Clostridium difficile PCR kit |
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PCR-VH005-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Clostridium difficile |
![]() Clostridium difficile Toxin A (C. difficile toxA) Antibody |
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abx411009-1ml | Abbexa | 1 ml | EUR 439 |
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![]() Clostridium difficile Toxin A (C. difficile toxA) Antibody |
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abx414381-01mg | Abbexa | 0.1 mg | EUR 578 |
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![]() Clostridium difficile Toxin B (C. difficile toxB) Antibody |
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abx414897-01mg | Abbexa | 0.1 mg | EUR 578 |
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![]() Clostridium difficile Toxin B (C. difficile toxB) Antibody |
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abx414898-01mg | Abbexa | 0.1 mg | EUR 439 |
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![]() Polyclonal NAP1 Antibody |
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APR17531G | Leading Biology | 0.1ml | EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NAP1 . This antibody is tested and proven to work in the following applications: |
![]() Nap1 Polyclonal Antibody |
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ES2896-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against Nap1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() Nap1 Polyclonal Antibody |
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ES2896-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against Nap1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA |
![]() NAP1 Blocking Peptide |
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20-abx063949 | Abbexa |
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![]() Nap1 Polyclonal Antibody |
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ABP51897-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of Nap1 from Human, Mouse, Rat. This Nap1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Nap1 at AA range: 10-90 |
![]() Nap1 Polyclonal Antibody |
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ABP51897-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of Nap1 from Human, Mouse, Rat. This Nap1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Nap1 at AA range: 10-90 |
![]() Nap1 Polyclonal Antibody |
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ABP51897-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of Nap1 from Human, Mouse, Rat. This Nap1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Nap1 at AA range: 10-90 |
![]() Anti-Nap1 antibody |
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STJ94343 | St John's Laboratory | 200 µl | EUR 197 |
Description: Rabbit polyclonal to Nap1. |
![]() Clostridium difficile Toxin A Antibody |
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abx120033-1ml | Abbexa | 1 ml | EUR 968 |
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![]() Clostridium difficile Toxin A Antibody |
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abx120034-1mg | Abbexa | 1 mg | EUR 878 |
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![]() Clostridium difficile Toxin B Antibody |
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abx120035-1ml | Abbexa | 1 ml | EUR 801 |
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![]() Clostridium difficile Toxin B Antibody |
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abx120036-1mg | Abbexa | 1 mg | EUR 662 |
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![]() Clostridium difficile Toxin A Antibody |
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abx021624-1mg | Abbexa | 1 mg | EUR 1358 |
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![]() Clostridium difficile Toxin A Antibody |
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abx021625-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile Toxin A Antibody |
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abx021626-1mg | Abbexa | 1 mg | EUR 1017 |
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![]() Clostridium difficile Toxin A Antibody |
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abx021627-1mg | Abbexa | 1 mg | EUR 1017 |
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![]() Clostridium difficile Toxin B Antibody |
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abx021628-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile Toxin B Antibody |
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abx021629-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile Toxin B Antibody |
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abx021630-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile Toxin B Antibody |
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abx021631-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile Toxin B Antibody |
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abx021632-1mg | Abbexa | 1 mg | EUR 996 |
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![]() Clostridium difficile toxin B antibody |
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10-1274 | Fitzgerald | 1 mg | EUR 873 |
Description: Mouse monoclonal Clostridium difficile toxin B antibody |
![]() Clostridium difficile toxin B antibody |
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10-1276 | Fitzgerald | 1 mg | EUR 989 |
Description: Mouse monoclonal Clostridium difficile toxin B antibody |
![]() Clostridium difficile Toxoid A protein |
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30-1861 | Fitzgerald | 500 ug | EUR 2253 |
Description: Purified Native Clostridium difficile Toxoid A protein |
![]() Clostridium difficile Toxoid B protein |
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30-1862 | Fitzgerald | 100 ug | EUR 1753 |
Description: Purified Native Clostridium difficile Toxoid B protein |
![]() Clostridium difficile Toxin A protein |
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30-1863 | Fitzgerald | 10 ug | EUR 1961 |
Description: Purified Native Clostridium difficile Toxin A protein (ribotype 027) |
![]() Clostridium difficile Toxin B protein |
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30-1864 | Fitzgerald | 10 ug | EUR 2252 |
Description: Purified Native Clostridium difficile Toxin B protein (ribotype 027) |
![]() Clostridium difficile Toxin A protein |
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30-1865 | Fitzgerald | 10 ug | EUR 2349 |
Description: Purified Native Clostridium difficile Toxin A protein (ribotype 078) |
![]() Clostridium difficile Toxin B protein |
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30-1866 | Fitzgerald | 10 ug | EUR 2638 |
Description: Purified Native Clostridium difficile Toxin B protein (ribotype 078) |
![]() Clostridium difficile Toxin A antibody |
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20-CR65 | Fitzgerald | 1 mg | EUR 291 |
Description: Rabbit polyclonal Clostridium difficile Toxin A antibody |
![]() Clostridium difficile Toxin A antibody |
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70-1004 | Fitzgerald | 500 ul | EUR 486 |
Description: Goat polyclonal Clostridium difficile Toxin A antibody |
![]() Clostridium difficile Toxin A antibody |
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70-1005 | Fitzgerald | 100 ug | EUR 755 |
Description: Affinity purified Chicken polyclonal Clostridium difficile Toxin A antibody |
![]() Clostridium difficile toxin B antibody |
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70-1017 | Fitzgerald | 100 ug | EUR 755 |
Description: Affinity purified chicken polyclonal Clostridium difficile toxin B antibody |
![]() Clostridium difficile RT PCR kit |
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RTq-VH005-100D | Bioingentech | 100T | EUR 628.5 |
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Description: A Real-Time PCR kit for detection of Clostridium difficile . |
![]() Clostridium difficile RT PCR kit |
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RTq-VH005-150D | Bioingentech | 150T | EUR 701 |
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Description: A Real-Time PCR kit for detection of Clostridium difficile . |
![]() Clostridium difficile RT PCR kit |
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RTq-VH005-50D | Bioingentech | 50T | EUR 532.8 |
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Description: A Real-Time PCR kit for detection of Clostridium difficile . |
![]() Clostridium Difficile Glutamate Dehydrogenase Antigen |
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VAng-Lsx9069-10g | Creative Biolabs | 10 µg | EUR 1407 |
Description: Clostridium Difficile Glutamate Dehydrogenase antigen from E. coli. |
![]() Inactivated Clostridium Difficile Toxoid A |
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VAng-Lsx2763-50g | Creative Biolabs | 50 µg | EUR 2800 |
Description: Inactivated toxoid derived from purified Clostridium Difficile Toxin A. |
![]() Inactivated Clostridium Difficile Toxoid B |
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VAng-Lsx2764-50g | Creative Biolabs | 50 µg | EUR 2800 |
Description: Inactivated toxoid derived from purified Clostridium Difficile Toxin B. |
![]() Mouse NAP1 shRNA Plasmid |
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20-abx978308 | Abbexa |
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![]() Rat NAP1 shRNA Plasmid |
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20-abx988902 | Abbexa |
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![]() Clostridium difficile One-Step PCR kit |
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Oneq-VH005-100D | Bioingentech | 100T | EUR 747.4 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Clostridium difficile. |
![]() Clostridium difficile One-Step PCR kit |
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Oneq-VH005-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Clostridium difficile. |
![]() Clostridium difficile One-Step PCR kit |
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Oneq-VH005-50D | Bioingentech | 50T | EUR 628.5 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Clostridium difficile. |
![]() Human Clostridium Difficile Toxin A+ B |
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QY-E05477 | Qayee Biotechnology | 96T | EUR 361 |
![]() Inactivated Clostridium Difficile Glutamate Dehydrogenase Antigen |
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VAng-Lsx2762-100g | Creative Biolabs | 100 µg | EUR 1407 |
Description: Clostridium Difficile Glutamate Dehydrogenase, purified antigen. |
![]() Inactivated Clostridium Difficile Toxin A Antigen |
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VAng-Lsx2765-50g | Creative Biolabs | 50 µg | EUR 1208 |
Description: Purified Native Clostridium Difficile Toxin A protein. |
![]() Inactivated Clostridium Difficile Toxin B Antigen |
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VAng-Lsx2766-50g | Creative Biolabs | 50 µg | EUR 3186 |
Description: Purified Native Clostridium Difficile Toxin B protein. |
![]() Clostridium difficile NAD-specific glutamate dehydrogenase (gluD) |
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1-CSB-EP335284DUNa0 | Cusabio |
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Description: Recombinant Clostridium difficile NAD-specific glutamate dehydrogenase(gluD) expressed in E.coli |
![]() NATtrol Clostridium difficile (Stool Matrix) (0.5 mL) |
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NATCDI-GP | Zeptometrix | 0.5 mL | EUR 93.68 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
![]() Purified Native Clostridium Difficile Toxoid A Antigen |
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VAng-Lsx9070-50g | Creative Biolabs | 50 µg | EUR 1208 |
Description: Clostridium Difficile Toxoid A Antigen, purified native protein from C. difficile. |
![]() Purified Native Clostridium Difficile Toxoid B Antigen |
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VAng-Lsx9071-50g | Creative Biolabs | 50 µg | EUR 3186 |
Description: Clostridium Difficile Toxoid B Antigen, purified native protein from C. difficile. |
![]() Recombinant Clostridium Difficile Enolase (aa 1-57) |
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VAng-Lsx9151-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2753 |
Description: Clostridium Difficile Enolase, recombinant protein. |
![]() Recombinant Clostridium Difficile Enolase (aa 1-57) |
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VAng-Lsx9151-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1864 |
Description: Clostridium Difficile Enolase, recombinant protein. |
![]() Recombinant Clostridium Difficile Enolase (aa 1-57) |
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VAng-Lsx9151-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1279 |
Description: Clostridium Difficile Enolase, recombinant protein. |
![]() Rabbit anti-Clostridium Difficile Toxin A antiserum (Native C. difficile toxin A immunogen) |
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CDTA11-S | Alpha Diagnostics | 100 ul | EUR 482 |
![]() Mouse anti Clostridium difficile Toxin A Monoclonal Antibody |
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DCAB-TJ103 | Creative Diagnostics | 1 mg | EUR 1294 |
![]() Mouse anti Clostridium difficile Toxin A Monoclonal Antibody |
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DCAB-TJ124 | Creative Diagnostics | 1 mg | EUR 1339 |
![]() Mouse anti Clostridium difficile Toxin A Monoclonal Antibody |
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DCAB-TJ126 | Creative Diagnostics | 1 mg | EUR 1339 |
![]() Clostridium Difficile Toxin A and B PCR kit |
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PCR-V039-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Clostridium Difficile Toxin A and B |
![]() Clostridium Difficile Toxin A and B PCR kit |
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PCR-V039-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Clostridium Difficile Toxin A and B |
![]() Human Clostridium Difficile Toxin B(TOXB) ELISA kit |
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QY-E05475 | Qayee Biotechnology | 96T | EUR 361 |
![]() Human Clostridium Difficile Toxin A(TOXA) ELISA kit |
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QY-E05476 | Qayee Biotechnology | 96T | EUR 361 |
![]() Recombinant Clostridium Difficile accD Protein (aa 1-284) |
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VAng-Lsx9072-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4215 |
Description: Clostridium Difficile (strain 630) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, recombinant protein. |
![]() Recombinant Clostridium Difficile accD Protein (aa 1-284) |
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VAng-Lsx9072-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2858 |
Description: Clostridium Difficile (strain 630) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, recombinant protein. |
![]() Recombinant Clostridium Difficile accD Protein (aa 1-284) |
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VAng-Lsx9072-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1945 |
Description: Clostridium Difficile (strain 630) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, recombinant protein. |
![]() Recombinant Clostridium Difficile ackA Protein (aa 1-398) |
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VAng-Lsx9073-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4941 |
Description: Clostridium Difficile (strain 630) Acetate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile ackA Protein (aa 1-398) |
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VAng-Lsx9073-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3338 |
Description: Clostridium Difficile (strain 630) Acetate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile ackA Protein (aa 1-398) |
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VAng-Lsx9073-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2273 |
Description: Clostridium Difficile (strain 630) Acetate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile addA Protein (aa 1-1275) |
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VAng-Lsx9074-inquire | Creative Biolabs | inquire | Ask for price |
Description: Clostridium Difficile (strain 630) ATP-dependent helicase/nuclease subunit A, recombinant protein. |
![]() Recombinant Clostridium Difficile addB Protein (aa 1-1155) |
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VAng-Lsx9075-inquire | Creative Biolabs | inquire | Ask for price |
Description: Clostridium Difficile (strain 630) ATP-dependent helicase/deoxyribonuclease subunit B, recombinant protein. |
![]() Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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VAng-Lsx9076-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3782 |
Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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VAng-Lsx9076-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2566 |
Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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VAng-Lsx9076-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1747 |
Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile alaS Protein (aa 1-879) |
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VAng-Lsx9077-inquire | Creative Biolabs | inquire | Ask for price |
Description: Clostridium Difficile (strain 630) Alanine--tRNA ligase, recombinant protein. |
![]() Recombinant Clostridium Difficile anmK Protein (aa 1-383) |
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VAng-Lsx9078-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4859 |
Description: Clostridium Difficile (strain 630) Anhydro-N-acetylmuramic acid kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile anmK Protein (aa 1-383) |
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VAng-Lsx9078-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3291 |
Description: Clostridium Difficile (strain 630) Anhydro-N-acetylmuramic acid kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile anmK Protein (aa 1-383) |
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VAng-Lsx9078-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2238 |
Description: Clostridium Difficile (strain 630) Anhydro-N-acetylmuramic acid kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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VAng-Lsx9079-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3502 |
Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
![]() Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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VAng-Lsx9079-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2367 |
Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
![]() Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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VAng-Lsx9079-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1618 |
Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
![]() Recombinant Clostridium Difficile argB Protein (aa 1-286) |
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VAng-Lsx9080-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4215 |
Description: Clostridium Difficile (strain 630) Acetylglutamate kinase, recombinant protein. |
![]() Recombinant Clostridium Difficile argB Protein (aa 1-286) |
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VAng-Lsx9080-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2858 |
Description: Clostridium Difficile (strain 630) Acetylglutamate kinase, recombinant protein. |
Differential sensitivity to beta-cell secretagogues in cultured rat pancreatic islets uncovered to human interleukin-1 beta.
- The early levels of insulin-dependent diabetes mellitus are characterised by a selective incapacity to secrete insulin in response to glucose, coupled to a greater response to nonnutrient secretagogues. The poor glucose response could also be a results of the autoimmune course of directed towards the beta-cells.
Albumin (Capture / Detection) Antibody
- Interleukin-1 (IL-1) has been prompt to be one potential mediator of immunological harm of the beta-cells. Within the current research we characterised the sensitivity of beta-cells to totally different secretagogues after human recombinant IL-1 beta (rIL-1 beta) publicity.
- Moreover, experiments had been carried out to make clear the biochemical mechanisms behind the faulty insulin response noticed in these islets. Rat pancreatic islets had been remoted and saved in tissue tradition (medium RPMI-1640 plus 10% calf serum) for five days.
SCCA1 (Capture / Detection) Antibody
- The islets had been subsequently uncovered to 60 pM human recombinant IL-1 beta throughout 48 h in the identical tradition circumstances as above and examined instantly after IL-1 publicity. The rIL-1 beta-treated islets confirmed a marked discount of glucose-stimulated insulin launch.
- Stimulation with arginine plus totally different glucose concentrations, and leucine plus glutamine partially counteracted the rIL-1 beta-induced discount of insulin launch. The actions of the glycolytic enzymes hexokinase, glucokinase, and glyceraldehyde 3-phosphate dehydrogenase, had been related in management and IL-1-exposed islets.
- Therapy with IL-1 additionally didn’t impair the actions of NADH+- and NADPH+-dependent glutamate dehydrogenase, glutamate-aspartate transaminase, glutamate-alanine transaminase, citrate synthase, and NAD+-linked isocitrate dehydrogenase.
- The oxidation of D-[6-14C]glucose and L-[U-14C]leucine had been decreased by 50% in IL-1-treated islets. Moreover, there was a big lower within the ratios of [2-14C]pyruvate oxidation/[1-14C]pyruvate decarboxylation and L-[U-14C]leucine oxidation/L-[1-14C]leucine decarboxylation, indicating that IL-1 decreases the proportion of generated acetyl-coenzyme-A residues present process oxidation.
- Nonetheless, within the presence of IL-1 there was a big improve in L-[U-14C]glutamate oxidation. These mixed observations recommend that publicity to IL-1 induces a preferential lower in glucose-mediated insulin launch and mitochondrial glucose metabolism.
- This mitochondrial dysfunction appears to mirror an impairment in proximal steps of the Krebs cycle. It’s conceivable that the IL-1-induced suppression and shift in islet metabolism will be an evidence for the beta-cell insensitivity to glucose noticed within the early phases of human and experimental insulin-dependent diabetes mellitus.
![]() SYBR Green I |
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B1747-25 | Biovision | EUR 1164 |
![]() SYBR Green I |
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B1747-5 | Biovision | EUR 387 |
![]() Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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W2601-1 | 101Bio | NULL | EUR 0 |
![]() Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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W2601-5 | 101Bio | NULL | EUR 0 |
![]() EZSolution? SYBR Green I |
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B1748-1 | Biovision | EUR 620 |
![]() EZSolution? SYBR Green I |
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B1748-500 | Biovision | EUR 430 |
![]() OPTIONAL GREEN FILTER (572NM) |
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GDBL-GREEN | CORNING | 1/pk | EUR 1052 |
Description: Lab Equipment; Axygen Branded EQ |
![]() SYBR Green qPCR Master Mix |
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HY-K0501 | MedChemExpress | 5 mL (500 rxns) | EUR 263 |
![]() 2x SYBR Green qPCR Master Mix |
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B21202 | Bimake | 5 mL | EUR 224 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
![]() 2x SYBR Green qPCR Master Mix |
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B21203 | Bimake | 25 mL | EUR 856 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
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