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Best Recombinant Human Isocitrate dehydrogenase
Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase.
- Mycobacterium tuberculosis (Mtb) has tailored its metabolism for persistence within the human macrophage. The diversifications are more likely to contain Mtb’s core middleman metabolism, whose enzymes have been little studied. The tricarboxylic acid cycle is predicted to yield precursors for vitality, lipids, amino acids, and heme.
- The genome sequence of Mtb H37Rv predicts the presence of a whole tricarboxylic acid cycle, however we not too long ago discovered that alpha-ketoglutarate dehydrogenase (KDH) exercise is missing in Mtb lysates.
Clostridium difficile Toxin A Antibody
- Right here we confirmed that citrate synthase, aconitase, isocitrate dehydrogenase, fumarase, malate dehydrogenase, and succinate dehydrogenase, however not KDH, are current, elevating the potential for separate oxidative and reductive half-cycles.
- As a possible hyperlink between the half-cycles, we discovered that Rv1248c, annotated as encoding SucA, the putative E1 part of KDH, as an alternative encodes alpha-ketoglutarate decarboxylase (Kgd) and produces succinic semialdehyde. Succinic semialdehyde dehydrogenase exercise was detected in Mtb lysates and recapitulated with recombinant proteins GabD1 (encoded by Rv0234c) and GabD2 (encoded by Rv1731).
DNA Polymerase for PAGE (with 2.5 mM dNTPs) Enzyme
- Kgd and GabD1 or GabD2 type an alternate pathway from alpha-ketoglutarate to succinate. Rv1248c, which is crucial or required for regular development of Mtb [Sassetti, C., Boyd, D. H. & Rubin, E. J. (2003) Mol. Microbiol 48, 77-84] is the primary gene proven to encode a Kgd. Kgd is missing in people and will signify a possible goal for chemotherapy of tuberculosis.
CLOSTRIDIUM DIFFICILE AGAR |
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| C03-109-10kg | Alphabiosciences | 10 kg | EUR 2094 |
CLOSTRIDIUM DIFFICILE AGAR |
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| C03-109-2kg | Alphabiosciences | 2kg | EUR 501.6 |
CLOSTRIDIUM DIFFICILE AGAR |
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| C03-109-500g | Alphabiosciences | 500 g | EUR 180 |
Clostridium difficile PCR kit |
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| PCR-VH005-96D | Bioingentech | 100T | EUR 686.4 |
Clostridium difficile Toxoid A |
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| abx670094-50ug | Abbexa | 50 ug | EUR 1262.4 |
Clostridium difficile Toxoid B |
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| abx670095-50ug | Abbexa | 50 ug | EUR 1262.4 |
Clostridium difficile Toxin A (C. difficile toxA) Antibody |
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| abx411009-1ml | Abbexa | 1 ml | EUR 526.8 |
Clostridium difficile Toxin A (C. difficile toxA) Antibody |
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| abx414381-01mg | Abbexa | 0.1 mg | EUR 693.6 |
Clostridium difficile Toxin B (C. difficile toxB) Antibody |
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| abx414897-01mg | Abbexa | 0.1 mg | EUR 693.6 |
Clostridium difficile Toxin B (C. difficile toxB) Antibody |
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| abx414898-01mg | Abbexa | 0.1 mg | EUR 526.8 |
Clostridium difficile Select Sup |
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| MED1552 | Scientific Laboratory Supplies | PK10 | EUR 182.4 |
Clostridium difficile RT PCR kit |
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| RTq-VH005-100D | Bioingentech | 100T | EUR 860.4 |
Clostridium difficile RT PCR kit |
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| RTq-VH005-150D | Bioingentech | 150T | EUR 969.6 |
Clostridium difficile RT PCR kit |
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| RTq-VH005-50D | Bioingentech | 50T | EUR 717.6 |
Clostridium difficile Toxin A protein |
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| 30-1863 | Fitzgerald | 10 ug | EUR 2353.2 |
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Description: Purified Native Clostridium difficile Toxin A protein (ribotype 027) |
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Clostridium difficile Toxin B protein |
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| 30-1864 | Fitzgerald | 10 ug | EUR 2702.4 |
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Description: Purified Native Clostridium difficile Toxin B protein (ribotype 027) |
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Clostridium difficile Toxin A protein |
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| 30-1865 | Fitzgerald | 10 ug | EUR 2818.8 |
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Description: Purified Native Clostridium difficile Toxin A protein (ribotype 078) |
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Clostridium difficile Toxin B protein |
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| 30-1866 | Fitzgerald | 10 ug | EUR 3165.6 |
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Description: Purified Native Clostridium difficile Toxin B protein (ribotype 078) |
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Clostridium difficile Toxin A Antibody |
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| abx120033-1ml | Abbexa | 1 ml | EUR 1161.6 |
Clostridium difficile Toxin A Antibody |
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| abx120034-1mg | Abbexa | 1 mg | EUR 1053.6 |
Clostridium difficile Toxin B Antibody |
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| abx120035-1ml | Abbexa | 1 ml | EUR 961.2 |
Clostridium difficile Toxin B Antibody |
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| abx120036-1mg | Abbexa | 1 mg | EUR 794.4 |
Clostridium difficile toxin B antibody |
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| 10-1274 | Fitzgerald | 1 mg | EUR 1047.6 |
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Description: Mouse monoclonal Clostridium difficile toxin B antibody |
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Clostridium difficile toxin B antibody |
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| 10-1276 | Fitzgerald | 1 mg | EUR 1186.8 |
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Description: Mouse monoclonal Clostridium difficile toxin B antibody |
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Clostridium difficile Toxin A antibody |
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| 20-CR65 | Fitzgerald | 1 mg | EUR 349.2 |
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Description: Rabbit polyclonal Clostridium difficile Toxin A antibody |
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Clostridium difficile Toxoid A protein |
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| 30-1861 | Fitzgerald | 500 ug | EUR 2703.6 |
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Description: Purified Native Clostridium difficile Toxoid A protein |
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Clostridium difficile Toxoid B protein |
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| 30-1862 | Fitzgerald | 100 ug | EUR 2103.6 |
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Description: Purified Native Clostridium difficile Toxoid B protein |
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Clostridium difficile Toxin A Antibody |
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| abx021624-1mg | Abbexa | 1 mg | EUR 1629.6 |
Clostridium difficile Toxin A Antibody |
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| abx021625-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin A Antibody |
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| abx021626-1mg | Abbexa | 1 mg | EUR 1220.4 |
Clostridium difficile Toxin A Antibody |
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| abx021627-1mg | Abbexa | 1 mg | EUR 1220.4 |
Clostridium difficile Toxin B Antibody |
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| abx021628-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin B Antibody |
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| abx021629-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin B Antibody |
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| abx021630-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin B Antibody |
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| abx021631-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin B Antibody |
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| abx021632-1mg | Abbexa | 1 mg | EUR 1195.2 |
Clostridium difficile Toxin A antibody |
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| 70-1004 | Fitzgerald | 500 ul | EUR 583.2 |
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Description: Goat polyclonal Clostridium difficile Toxin A antibody |
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Clostridium difficile Toxin A antibody |
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| 70-1005 | Fitzgerald | 100 ug | EUR 906 |
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Description: Affinity purified Chicken polyclonal Clostridium difficile Toxin A antibody |
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Clostridium difficile toxin B antibody |
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| 70-1017 | Fitzgerald | 100 ug | EUR 906 |
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Description: Affinity purified chicken polyclonal Clostridium difficile toxin B antibody |
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Clostridium difficile Toxin A Antibody |
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| V8285-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
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Description: This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation. |
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Clostridium difficile Toxin A Antibody |
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| V8285-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
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Description: This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation. |
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Clostridium difficile Toxin A Antibody |
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| V8285SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
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Description: This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation. |
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Human Clostridium Difficile Toxin A+ B |
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| QY-E05477 | Qayee Biotechnology | 96T | EUR 433.2 |
Clostridium difficile One-Step PCR kit |
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| Oneq-VH005-100D | Bioingentech | 100T | EUR 1039.2 |
Clostridium difficile One-Step PCR kit |
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| Oneq-VH005-150D | Bioingentech | 150T | EUR 1177.2 |
Clostridium difficile One-Step PCR kit |
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| Oneq-VH005-50D | Bioingentech | 50T | EUR 861.6 |
Inactivated Clostridium Difficile Toxoid A |
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| VAng-Lsx2763-50g | Creative Biolabs | 50 µg | EUR 3360 |
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Description: Inactivated toxoid derived from purified Clostridium Difficile Toxin A. |
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Inactivated Clostridium Difficile Toxoid B |
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| VAng-Lsx2764-50g | Creative Biolabs | 50 µg | EUR 3360 |
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Description: Inactivated toxoid derived from purified Clostridium Difficile Toxin B. |
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Clostridium Difficile Toxin A and B PCR kit |
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| PCR-V039-48D | Bioingentech | 50T | EUR 543.6 |
Clostridium Difficile Toxin A and B PCR kit |
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| PCR-V039-96D | Bioingentech | 100T | EUR 686.4 |
Clostridium Difficile Toxin A and B RT PCR kit |
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| RTq-V039-100D | Bioingentech | 100T | EUR 860.4 |
Clostridium Difficile Toxin A and B RT PCR kit |
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| RTq-V039-150D | Bioingentech | 150T | EUR 969.6 |
Clostridium Difficile Toxin A and B RT PCR kit |
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| RTq-V039-50D | Bioingentech | 50T | EUR 717.6 |
NATtrol Clostridium difficile (Stool Matrix) (0.5 mL) |
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| NATCDI-GP | Zeptometrix | 0.5 mL | EUR 55 |
Inactivated Clostridium Difficile Toxin A Antigen |
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| VAng-Lsx2765-50g | Creative Biolabs | 50 µg | EUR 1449.6 |
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Description: Purified Native Clostridium Difficile Toxin A protein. |
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Inactivated Clostridium Difficile Toxin B Antigen |
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| VAng-Lsx2766-50g | Creative Biolabs | 50 µg | EUR 3823.2 |
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Description: Purified Native Clostridium Difficile Toxin B protein. |
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Recombinant Clostridium Difficile Enolase (aa 1-57) |
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| VAng-Lsx9151-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3303.6 |
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Description: Clostridium Difficile Enolase, recombinant protein. |
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Recombinant Clostridium Difficile Enolase (aa 1-57) |
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| VAng-Lsx9151-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2236.8 |
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Description: Clostridium Difficile Enolase, recombinant protein. |
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Recombinant Clostridium Difficile Enolase (aa 1-57) |
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| VAng-Lsx9151-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1534.8 |
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Description: Clostridium Difficile Enolase, recombinant protein. |
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Clostridium Difficile Glutamate Dehydrogenase Antigen |
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| VAng-Lsx9069-10g | Creative Biolabs | 10 µg | EUR 1688.4 |
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Description: Clostridium Difficile Glutamate Dehydrogenase antigen from E. coli. |
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Human Clostridium Difficile Toxin B（TOXB) ELISA kit |
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| QY-E05475 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Clostridium Difficile Toxin A(TOXA) ELISA kit |
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| QY-E05476 | Qayee Biotechnology | 96T | EUR 433.2 |
Recombinant Clostridium Difficile Toxoid A (aa 1-2710) |
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| VAng-Lsx9372-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6855.6 |
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Description: Clostridium Difficile Toxoid A, recombinant protein. |
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Recombinant Clostridium Difficile Toxoid A (aa 1-2710) |
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| VAng-Lsx9372-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4636.8 |
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Description: Clostridium Difficile Toxoid A, recombinant protein. |
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Recombinant Clostridium Difficile Toxoid A (aa 1-2710) |
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| VAng-Lsx9372-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 3148.8 |
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Description: Clostridium Difficile Toxoid A, recombinant protein. |
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Recombinant Clostridium Difficile Toxoid B (aa 1-2366) |
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| VAng-Lsx9373-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6855.6 |
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Description: Clostridium Difficile Toxoid B, recombinant protein. |
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Recombinant Clostridium Difficile Toxoid B (aa 1-2366) |
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| VAng-Lsx9373-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4636.8 |
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Description: Clostridium Difficile Toxoid B, recombinant protein. |
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Recombinant Clostridium Difficile Toxoid B (aa 1-2366) |
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| VAng-Lsx9373-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 3148.8 |
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Description: Clostridium Difficile Toxoid B, recombinant protein. |
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Native Clostridium Difficile Toxoid A (VPI 10463, >95% Pure) |
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| CDTA15-R-25 | Alpha Diagnostics | 25 ug | EUR 548.4 |
Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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| VAng-Lsx9076-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4538.4 |
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Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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| VAng-Lsx9076-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3079.2 |
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Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile adk Protein (aa 1-216) |
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| VAng-Lsx9076-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2096.4 |
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Description: Clostridium Difficile (strain 630) Adenylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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| VAng-Lsx9079-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4202.4 |
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Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
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Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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| VAng-Lsx9079-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2840.4 |
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Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
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Recombinant Clostridium Difficile apt Protein (aa 1-170) |
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| VAng-Lsx9079-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1941.6 |
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Description: Clostridium Difficile (strain 630) Adenine phosphoribosyltransferase, recombinant protein. |
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Recombinant Clostridium Difficile cmk Protein (aa 1-216) |
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| VAng-Lsx9125-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4538.4 |
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Description: Clostridium Difficile (strain 630) Cytidylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile cmk Protein (aa 1-216) |
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| VAng-Lsx9125-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3079.2 |
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Description: Clostridium Difficile (strain 630) Cytidylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile cmk Protein (aa 1-216) |
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| VAng-Lsx9125-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2096.4 |
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Description: Clostridium Difficile (strain 630) Cytidylate kinase, recombinant protein. |
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Recombinant Clostridium Difficile crt Protein (aa 1-155) |
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| VAng-Lsx9130-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4075.2 |
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Description: Clostridium Difficile 3-hydroxybutyryl-CoA dehydratase, recombinant protein. |
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Recombinant Clostridium Difficile crt Protein (aa 1-155) |
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| VAng-Lsx9130-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2755.2 |
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Description: Clostridium Difficile 3-hydroxybutyryl-CoA dehydratase, recombinant protein. |
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Recombinant Clostridium Difficile crt Protein (aa 1-155) |
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| VAng-Lsx9130-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1885.2 |
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Description: Clostridium Difficile 3-hydroxybutyryl-CoA dehydratase, recombinant protein. |
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Recombinant Clostridium Difficile dtd Protein (aa 1-149) |
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| VAng-Lsx9141-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4018.8 |
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Description: Clostridium Difficile (strain 630) D-tyrosyl-tRNA (Tyr) deacylase, recombinant protein. |
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Recombinant Clostridium Difficile dtd Protein (aa 1-149) |
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| VAng-Lsx9141-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2713.2 |
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Description: Clostridium Difficile (strain 630) D-tyrosyl-tRNA (Tyr) deacylase, recombinant protein. |
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Recombinant Clostridium Difficile dtd Protein (aa 1-149) |
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| VAng-Lsx9141-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1857.6 |
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Description: Clostridium Difficile (strain 630) D-tyrosyl-tRNA (Tyr) deacylase, recombinant protein. |
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Recombinant Clostridium Difficile dut Protein (aa 1-143) |
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| VAng-Lsx9142-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3976.8 |
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Description: Clostridium Difficile Deoxyuridine 5'-triphosphate nucleotidohydrolase, recombinant protein. |
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Recombinant Clostridium Difficile dut Protein (aa 1-143) |
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| VAng-Lsx9142-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2700 |
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Description: Clostridium Difficile Deoxyuridine 5'-triphosphate nucleotidohydrolase, recombinant protein. |
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Recombinant Clostridium Difficile dut Protein (aa 1-143) |
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| VAng-Lsx9142-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1843.2 |
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Description: Clostridium Difficile Deoxyuridine 5'-triphosphate nucleotidohydrolase, recombinant protein. |
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Recombinant Clostridium Difficile dxr Protein (aa 1-384) |
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| VAng-Lsx9144-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5830.8 |
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Description: Clostridium Difficile (strain 630) 1-deoxy-D-xylulose 5-phosphate reductoisomerase, recombinant protein. |
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Recombinant Clostridium Difficile dxr Protein (aa 1-384) |
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| VAng-Lsx9144-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3949.2 |
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Description: Clostridium Difficile (strain 630) 1-deoxy-D-xylulose 5-phosphate reductoisomerase, recombinant protein. |
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Recombinant Clostridium Difficile dxr Protein (aa 1-384) |
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| VAng-Lsx9144-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2685.6 |
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Description: Clostridium Difficile (strain 630) 1-deoxy-D-xylulose 5-phosphate reductoisomerase, recombinant protein. |
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Recombinant Clostridium Difficile dxs Protein (aa 1-621) |
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| VAng-Lsx9145-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Clostridium Difficile (strain 630) 1-deoxy-D-xylulose-5-phosphate synthase, recombinant protein. |
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Recombinant Clostridium Difficile efp Protein (aa 1-185) |
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| VAng-Lsx9148-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4257.6 |
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Description: Clostridium Difficile (strain 630) Elongation factor P, recombinant protein. |
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Recombinant Clostridium Difficile efp Protein (aa 1-185) |
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| VAng-Lsx9148-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2882.4 |
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Description: Clostridium Difficile (strain 630) Elongation factor P, recombinant protein. |
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Recombinant Clostridium Difficile efp Protein (aa 1-185) |
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| VAng-Lsx9148-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1969.2 |
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Description: Clostridium Difficile (strain 630) Elongation factor P, recombinant protein. |
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Recombinant Clostridium Difficile eno Protein (aa 1-430) |
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| VAng-Lsx9150-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6210 |
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Description: Clostridium Difficile (strain 630) Enolase, recombinant protein. |
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Recombinant Clostridium Difficile eno Protein (aa 1-430) |
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| VAng-Lsx9150-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4202.4 |
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Description: Clostridium Difficile (strain 630) Enolase, recombinant protein. |
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Recombinant Clostridium Difficile eno Protein (aa 1-430) |
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| VAng-Lsx9150-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2853.6 |
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Description: Clostridium Difficile (strain 630) Enolase, recombinant protein. |
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Recombinant Clostridium Difficile era Protein (aa 1-297) |
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| VAng-Lsx9152-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5156.4 |
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Description: Clostridium Difficile (strain 630) GTPase Era, recombinant protein. |
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Recombinant Clostridium Difficile era Protein (aa 1-297) |
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| VAng-Lsx9152-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3486 |
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Description: Clostridium Difficile (strain 630) GTPase Era, recombinant protein. |
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Recombinant Clostridium Difficile era Protein (aa 1-297) |
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| VAng-Lsx9152-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2377.2 |
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Description: Clostridium Difficile (strain 630) GTPase Era, recombinant protein. |
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Recombinant Clostridium Difficile fbp Protein (aa 1-661) |
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| VAng-Lsx9156-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Clostridium Difficile (strain 630) Fructose-1,6-bisphosphatase class 3, recombinant protein. |
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Differential sensitivity to beta-cell secretagogues in cultured rat pancreatic islets uncovered to human interleukin-1 beta.
- The early levels of insulin-dependent diabetes mellitus are characterised by a selective incapacity to secrete insulin in response to glucose, coupled to a greater response to nonnutrient secretagogues. The poor glucose response could also be a results of the autoimmune course of directed towards the beta-cells.
Albumin (Capture / Detection) Antibody
- Interleukin-1 (IL-1) has been prompt to be one potential mediator of immunological harm of the beta-cells. Within the current research we characterised the sensitivity of beta-cells to totally different secretagogues after human recombinant IL-1 beta (rIL-1 beta) publicity.
- Moreover, experiments had been carried out to make clear the biochemical mechanisms behind the faulty insulin response noticed in these islets. Rat pancreatic islets had been remoted and saved in tissue tradition (medium RPMI-1640 plus 10% calf serum) for five days.
SCCA1 (Capture / Detection) Antibody
- The islets had been subsequently uncovered to 60 pM human recombinant IL-1 beta throughout 48 h in the identical tradition circumstances as above and examined instantly after IL-1 publicity. The rIL-1 beta-treated islets confirmed a marked discount of glucose-stimulated insulin launch.
- Stimulation with arginine plus totally different glucose concentrations, and leucine plus glutamine partially counteracted the rIL-1 beta-induced discount of insulin launch. The actions of the glycolytic enzymes hexokinase, glucokinase, and glyceraldehyde 3-phosphate dehydrogenase, had been related in management and IL-1-exposed islets.
- Therapy with IL-1 additionally didn’t impair the actions of NADH+- and NADPH+-dependent glutamate dehydrogenase, glutamate-aspartate transaminase, glutamate-alanine transaminase, citrate synthase, and NAD+-linked isocitrate dehydrogenase.
- The oxidation of D-[6-14C]glucose and L-[U-14C]leucine had been decreased by 50% in IL-1-treated islets. Moreover, there was a big lower within the ratios of [2-14C]pyruvate oxidation/[1-14C]pyruvate decarboxylation and L-[U-14C]leucine oxidation/L-[1-14C]leucine decarboxylation, indicating that IL-1 decreases the proportion of generated acetyl-coenzyme-A residues present process oxidation.
- Nonetheless, within the presence of IL-1 there was a big improve in L-[U-14C]glutamate oxidation. These mixed observations recommend that publicity to IL-1 induces a preferential lower in glucose-mediated insulin launch and mitochondrial glucose metabolism.
- This mitochondrial dysfunction appears to mirror an impairment in proximal steps of the Krebs cycle. It’s conceivable that the IL-1-induced suppression and shift in islet metabolism will be an evidence for the beta-cell insensitivity to glucose noticed within the early phases of human and experimental insulin-dependent diabetes mellitus.
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