Pierce Renilla Firefly Luciferase Dual Assay Kit 16185

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.

Description: Minimum order quantity: 1 unit of 50AS

Description: Minimum order quantity: 1 unit of 150TST

Description: Minimum order quantity: 1 unit of 1000TST

Description: Minimum order quantity: 1 unit of 150AS

Description: Minimum order quantity: 1 unit of 1000AS

Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Description: Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology. [Wiki]

Description: Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology. [Wiki]